Year: 2016 | Month: December | Volume 6 | Issue 2
Enhanced Ethanol Production Through Salt Pre-conditioning of S.cerevisiae MTCC 11815
Abstract:
Salt preconditioning provides an alternate to very high gravity fermentation for increasing osmotolerance and thermotolerance of yeast. In the current study, a fermenting yeast strain (Saccharomyces cerevisiae MTCC 11815) was sequentially preconditioned at 4% (w/v) NaCl in a synthetic medium and optimized using RSM on the basis of osmotolerance and thermotolerance for maximum ethanol production. A maximum ethanol of 12.53% (v/v) was observed at a temperature of 35ºC with high residual Brix of 17 °B from initial 39 °B. Further, ethanol fermentation carried out between 20 to 30 °B revealed 24 °B as optimum with an ethanol production of 12.4 % (v/v) and residual brix of 3.5 °B. Validation studies on sugarcane juice as well as on molasses (10L) recorded the optimized fermentation parameters (Brix 24°B, Inoculum size 7.5% (v/v) and DAHP 0.3% (w/v)) with ethanol production of 12.4% (v/v) on sugar cane juice and 10.6% (v/v) on molasses having fermentation efficiencies of 86.10% and 81.20% by pre-conditioned S.cerevisiae MTCC 11815 cells.
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